Chemistry · Differentiations & Conversions (Organic)

Distinguishing Tests — Tollens' & Fehling's (Aldehydes vs Ketones)

Aldehydes and ketones share the same carbonyl group, yet a single drop of the right reagent separates them cleanly. Tollens' and Fehling's tests exploit the fact that an aldehyde is readily oxidised while a ketone resists it — the core idea developed in the NCERT Class 12 chapter on aldehydes, ketones and carboxylic acids and in the NIOS unit on the chemistry of carbonyl compounds. For NEET, the question is rarely "what is the product"; it is "which compound gives a silver mirror, which gives a brick-red precipitate, and why".

Why aldehydes and ketones diverge

Both aldehydes ($\ce{RCHO}$) and ketones ($\ce{R2CO}$) contain a carbonyl group, and most addition reactions of the carbonyl carbon are common to both. The decisive difference appears in oxidation. In an aldehyde, the carbonyl carbon carries a hydrogen atom; this aldehydic $\ce{C-H}$ bond can be replaced by a $\ce{C-OH}$ on oxidation, converting the aldehyde to a carboxylic acid (or its salt in alkaline medium) without breaking any carbon–carbon bond.

A ketone has no such hydrogen — the carbonyl carbon is flanked by two carbon groups. To oxidise a ketone you must rupture a strong $\ce{C-C}$ bond, which only harsh oxidants achieve and which always degrades the molecule. The mild, alkaline reagents used in distinguishing tests cannot do this. Consequently:

Aldehydes are oxidised by mild oxidising agents and therefore behave as reducing agents — they reduce $\ce{Ag+}$ to silver and $\ce{Cu^2+}$ to $\ce{Cu+}$. Ketones do not, so they leave these reagents unchanged. This single asymmetry is the engine behind Tollens', Fehling's and Benedict's tests.

NEET Trap

"Both have a carbonyl, so both reduce Tollens'."

A common error is to treat aldehydes and ketones as interchangeable because they share $\ce{>C=O}$. The carbonyl governs addition chemistry, not oxidation. Only the aldehyde's $\ce{C-H}$ makes it a reducing agent.

Rule: mild oxidation distinguishes them — aldehyde reduces the reagent, ketone does not.

Tollens' test: the silver mirror

Tollens' reagent is ammoniacal silver nitrate — the colourless diamminesilver(I) complex $\ce{[Ag(NH3)2]+}$. It must be prepared fresh just before use. Adding a little dilute $\ce{NaOH}$ to silver nitrate first throws down a brown precipitate of silver oxide; aqueous ammonia is then added dropwise until that precipitate just dissolves to a clear solution:

$$\ce{2AgNO3 + 2NaOH -> Ag2O v + 2NaNO3 + H2O}$$

$$\ce{Ag2O + 4NH3 + H2O -> 2[Ag(NH3)2]OH}$$

When an aldehyde is warmed gently with this reagent, the silver(I) is reduced to metallic silver, which deposits as a bright, mirror-like film on the clean inner wall of the tube — the famous silver mirror. The aldehyde is oxidised to the carboxylate ion (the medium is basic):

$$\ce{RCHO + 2[Ag(NH3)2]+ + 3OH- -> RCOO- + 2Ag v + 4NH3 + 2H2O}$$

Ketones give no reaction — the solution stays clear and no silver deposits. The test is therefore positive for aldehydes and negative for ketones. Importantly, both aliphatic and aromatic aldehydes respond: benzaldehyde gives a positive Tollens' test even though it fails Fehling's.

Figure 1 · Silver-mirror visual Aldehyde Silver mirror — POSITIVE Ketone Clear — NEGATIVE Ammoniacal AgNO₃, warm gently

Fehling's test: the brick-red precipitate

Fehling's solution comes in two bottles that are mixed in equal volumes immediately before use:

ComponentContentsRole
Fehling AAqueous copper(II) sulphate, CuSO₄Source of the oxidising Cu(II)
Fehling BAlkaline sodium potassium tartrate (Rochelle salt) in NaOHKeeps Cu(II) in solution; provides alkali

On mixing, the tartrate chelates copper(II) to give a deep-blue complex; this stops $\ce{Cu(OH)2}$ from precipitating prematurely in the alkaline medium. When an aldehyde is warmed with this blue solution, the copper(II) is reduced to copper(I), which separates as a brick-red precipitate of cuprous oxide, $\ce{Cu2O}$. The disappearance of blue and appearance of red is the positive signal:

$$\ce{RCHO + 2Cu^2+ + 5OH- -> RCOO- + Cu2O v + 3H2O}$$

The selectivity of Fehling's is sharper than Tollens'. Aliphatic aldehydes give a positive test; aromatic aldehydes (e.g. benzaldehyde) generally do not reduce Fehling's solution, and ketones are negative. This makes the pair of tests jointly diagnostic, as the comparison table below shows.

Figure 2 · Colour change in Fehling's test Deep blue Cu(II) tartrate + RCHO, warm Brick-red Cu₂O precipitate
Related distinguishing test

Methyl ketones and ethanal also answer to a colour-and-precipitate test of their own — see the Iodoform test for the yellow $\ce{CHI3}$ that separates $\ce{CH3CO-}$ compounds.

Benedict's test (the sugar variant)

Benedict's reagent is a single, more stable solution containing copper(II) sulphate, sodium citrate and sodium carbonate. The citrate plays the same stabilising role as the tartrate in Fehling's, but the one-bottle formulation keeps better, which is why Benedict's is preferred for routine detection of reducing sugars. The chemistry mirrors Fehling's: an aldehyde (or a reducing sugar) reduces the blue $\ce{Cu^2+}$ to a brick-red $\ce{Cu2O}$ precipitate. For NEET it is enough to know that Benedict's is the milder, citrate-based analogue of Fehling's and gives the same brick-red result with reducing sugars.

The redox basis of all three

All three tests are the same redox event seen through different metal ions. The aldehyde is the reducing agent: it is oxidised from the aldehyde to the carboxylate, surrendering electrons. The metal ion is the oxidising agent: it is reduced and reports the result as a visible product — a silver mirror or a red oxide.

TestOxidising species (reduced)Visible productAldehyde fate
Tollens'$\ce{Ag+}$ → $\ce{Ag^0}$Silver mirror$\ce{RCHO -> RCOO-}$
Fehling's$\ce{Cu^2+}$ → $\ce{Cu+}$Brick-red $\ce{Cu2O}$$\ce{RCHO -> RCOO-}$
Benedict's$\ce{Cu^2+}$ → $\ce{Cu+}$Brick-red $\ce{Cu2O}$$\ce{RCHO -> RCOO-}$

The half-reaction for the aldehyde is the same in every case; in alkaline medium it is written as the oxidation of the aldehyde to the carboxylate ion, $\ce{RCHO + 3OH- -> RCOO- + 2H2O + 2e-}$. The two electrons released reduce two $\ce{Ag+}$ to silver, or reduce $\ce{Cu^2+}$ to $\ce{Cu+}$ which then appears as $\ce{Cu2O}$.

What tells aldehyde from ketone

The two tests, read together, classify almost any carbonyl an examiner will offer. The table below is the one to memorise.

Compound classTollens' (silver mirror)Fehling's (brick-red Cu₂O)
Aliphatic aldehyde (e.g. ethanal)PositivePositive
Aromatic aldehyde (e.g. benzaldehyde)PositiveNegative (generally)
Ketone (e.g. propanone)NegativeNegative
Formic acid / formatePositivePositive
Reducing sugar (glucose, fructose)PositivePositive
Sucrose (non-reducing)NegativeNegative

The decision logic follows directly: a silver mirror confirms an aldehyde; if the same sample also gives brick-red with Fehling's it is aliphatic, and if it fails Fehling's it is aromatic. A compound that is negative to both Tollens' and Fehling's is a ketone (or a non-reducing species).

Figure 3 · Decision flow Unknown carbonyl Tollens' → silver mirror? No Ketone (non-reducing) Yes Fehling's → brick-red? Yes Aliphatic aldehyde No Aromatic aldehyde

Worked distinguishing examples

Example 1

Distinguish ethanal ($\ce{CH3CHO}$) from propanone ($\ce{CH3COCH3}$) chemically.

Warm each separately with Tollens' reagent. Ethanal, an aldehyde, reduces $\ce{[Ag(NH3)2]+}$ and deposits a silver mirror; propanone, a ketone, gives no change. The reaction with ethanal:

$$\ce{CH3CHO + 2[Ag(NH3)2]+ + 3OH- -> CH3COO- + 2Ag v + 4NH3 + 2H2O}$$

A confirmatory Fehling's test would give a brick-red $\ce{Cu2O}$ with ethanal and nothing with propanone. (Note: both ethanal and propanone also give a positive iodoform test, so iodoform alone cannot tell them apart — only an oxidation test can.)

Example 2

Benzaldehyde and acetophenone ($\ce{C6H5COCH3}$) are both colourless liquids. How do you distinguish them?

Use Tollens' reagent. Benzaldehyde, being an aldehyde, gives a silver mirror; acetophenone, a ketone, gives no mirror. Fehling's would not help separate them here, because benzaldehyde (aromatic aldehyde) generally fails Fehling's just as the ketone does. So Tollens' is the decisive test.

$$\ce{C6H5CHO + 2[Ag(NH3)2]+ + 3OH- -> C6H5COO- + 2Ag v + 4NH3 + 2H2O}$$

Example 3

A sample gives a silver mirror with Tollens' but no brick-red precipitate with Fehling's. Identify the class.

Positive Tollens' rules out a ketone and confirms an aldehyde. A negative Fehling's points to an aromatic aldehyde such as benzaldehyde, since aliphatic aldehydes would have given the brick-red $\ce{Cu2O}$ as well.

NEET Trap

Tollens' reagent must be freshly prepared.

On standing, ammoniacal silver nitrate can form explosive silver nitride ("fulminating silver"). Examiners often test the procedural fact that the reagent is made fresh and not stored, and that the test tube must be scrupulously clean for the mirror to form.

Remember: Tollens' = ammoniacal AgNO₃, prepared fresh; warm gently, do not boil hard.

Special cases & reducing sugars

A few species behave as aldehydes even though they are not simple $\ce{RCHO}$ molecules, and they recur in NEET options:

SpeciesBehaviourReason
Formic acid, $\ce{HCOOH}$Positive Tollens' and Fehling'sRetains an aldehydic $\ce{-CHO}$-like H on the carbon; acts as a reducing agent
Glucose, fructosePositive (reducing sugars)Free or potential carbonyl that can open to an aldehyde/α-hydroxy ketone form
Maltose, lactosePositive (reducing sugars)Have a free anomeric –OH that opens to a reducing aldehyde
SucroseNegative (non-reducing)Both anomeric carbons are locked in the glycosidic bond

Note that fructose is a ketose yet still reduces Tollens' and Fehling's: under the basic conditions of the test it isomerises to glucose/mannose (an aldose), which then reduces the reagent. This is why "ketone → always negative" must be read carefully — it applies to ordinary ketones, not to α-hydroxy ketones such as reducing sugars in alkaline medium.

Quick Recap

Tollens' & Fehling's in one screen

  • Why: aldehydes have a $\ce{C-H}$ on the carbonyl and act as reducing agents; ketones lack it and do not react with mild oxidants.
  • Tollens': ammoniacal $\ce{AgNO3}$, $\ce{[Ag(NH3)2]+}$, prepared fresh → silver mirror; positive for all aldehydes (aliphatic and aromatic), negative for ketones.
  • Fehling's: A = $\ce{CuSO4}$, B = Rochelle salt + $\ce{NaOH}$ → brick-red $\ce{Cu2O}$; positive for aliphatic aldehydes, generally negative for aromatic aldehydes and ketones.
  • Benedict's: citrate-based one-bottle analogue of Fehling's; same brick-red result, preferred for reducing sugars.
  • Redox: aldehyde is oxidised to the carboxylate; the metal ion ($\ce{Ag+}$ or $\ce{Cu^2+}$) is reduced and reports the result.
  • Watch: formic acid and reducing sugars are positive; sucrose is negative; benzaldehyde is positive to Tollens' but not Fehling's.

NEET PYQ Snapshot — Tollens' & Fehling's Tests

No NEET PYQ in the current bank targets these distinguishing tests directly, so the cards below are concept drills built on standard NCERT facts. Practise the reasoning, not a remembered year.

Concept

Which one of the following will NOT give a positive Tollens' test?

  1. Ethanal
  2. Benzaldehyde
  3. Propanone
  4. Formic acid
Answer: (3) Propanone

Propanone is a ketone with no aldehydic hydrogen, so it cannot be oxidised by mild Tollens' reagent and gives no silver mirror. Ethanal and benzaldehyde are aldehydes, and formic acid retains a reducing $\ce{-CHO}$-type hydrogen — all three are positive.

Concept

A carbonyl compound gives a silver mirror with Tollens' reagent but does not give a brick-red precipitate with Fehling's solution. The compound is most likely:

  1. Acetone
  2. Benzaldehyde
  3. Acetaldehyde
  4. Acetophenone
Answer: (2) Benzaldehyde

Positive Tollens' confirms an aldehyde (eliminates the two ketones). A negative Fehling's then points to an aromatic aldehyde — benzaldehyde — since the aliphatic acetaldehyde would also give brick-red $\ce{Cu2O}$.

Concept

Which reagent is used to prepare Fehling's solution B?

  1. Aqueous copper(II) sulphate only
  2. Sodium potassium tartrate (Rochelle salt) in NaOH
  3. Ammoniacal silver nitrate
  4. Sodium citrate in Na₂CO₃
Answer: (2) Rochelle salt in NaOH

Fehling A is aqueous $\ce{CuSO4}$; Fehling B is alkaline sodium potassium tartrate (Rochelle salt) in $\ce{NaOH}$. Option (4) describes Benedict's reagent; option (3) is Tollens' reagent.

FAQs — Tollens' & Fehling's Tests

Frequently confused points on the silver-mirror and brick-red tests.

Why do aldehydes give a positive Tollens' test but ketones do not?
The aldehydic hydrogen lets the carbonyl carbon be oxidised to a carboxylate without breaking a C–C bond, so the aldehyde reduces the silver(I) of Tollens' reagent to metallic silver. A ketone has two carbon groups flanking the carbonyl and no such hydrogen; its oxidation would require rupturing a strong C–C bond, which the mild reagent cannot do, so no silver mirror forms.
Does benzaldehyde give a positive Fehling's test?
No. Aromatic aldehydes such as benzaldehyde generally do not reduce Fehling's solution, so the test is effectively negative for them. They do, however, give a positive Tollens' test (silver mirror). This split is the standard way NEET distinguishes an aromatic aldehyde from an aliphatic one.
What is the composition of Tollens' reagent and how is it prepared?
Tollens' reagent is ammoniacal silver nitrate — the diamminesilver(I) ion, [Ag(NH3)2]+. It is prepared fresh by adding dilute NaOH to silver nitrate to precipitate brown silver oxide, then dissolving that precipitate by adding aqueous ammonia dropwise until the solution just clears.
What are Fehling A and Fehling B?
Fehling A is aqueous copper(II) sulphate. Fehling B is an alkaline solution of sodium potassium tartrate (Rochelle salt) in sodium hydroxide. Equal volumes are mixed just before use to give a deep-blue complex of Cu(II); the tartrate keeps copper in solution and prevents premature precipitation of copper hydroxide.
Why does Fehling's solution turn brick-red on a positive test?
The aldehyde reduces the deep-blue copper(II) complex to copper(I), which separates as a brick-red precipitate of cuprous oxide, Cu2O. The disappearance of the blue colour and appearance of the red solid is the positive signal.
Which test should be used to detect a reducing sugar?
Reducing sugars such as glucose, fructose, maltose and lactose contain a free or potential aldehyde/ketone group and give positive Tollens', Fehling's and Benedict's tests. Benedict's reagent is the usual choice for sugars because it is a single, more stable solution. Sucrose is non-reducing and gives all three negative.